Farm Animal Serum Proteomics and Impact on Human Health

Due to the incompleteness of animal genome sequencing, the analysis and characterization of serum proteomes of most farm animals are still in their infancy, compared to the already well-documented human serum proteome. This review focuses on the implications of the farm animal serum proteomics in order to identify novel biomarkers for animal welfare, early diagnosis, prognosis and monitoring of infectious disease treatment, and develop new vaccines, aiming at determining the reciprocal benefits for humans and animals.     

Anticancer Ruthenium(III) Complex KP1019 Interferes with ATP‐Dependent Ca2+ Translocation by Sarco‐Endoplasmic Reticulum Ca2+‐ATPase (SERCA)

Sarco‐endoplasmic reticulum Ca²⁺‐ATPase (SERCA), a P‐type ATPase that sustains Ca²⁺ transport and plays a major role in intracellular Ca²⁺ homeostasis, represents a therapeutic target for cancer therapy. Here, we investigated whether ruthenium‐based anticancer drugs, namely KP1019 (indazolium [trans‐tetrachlorobis(1H‐indazole)ruthenate(III)]), NAMI‐A (imidazolium [trans‐tetrachloro(1H‐imidazole)(S‐dimethylsulfoxide)ruthenate(III)]) and RAPTA‐C ([Ru(η⁶‐p‐cymene)dichloro(1,3,5‐triaza‐7‐phosphaadamantane)]), and cisplatin (cis‐diammineplatinum(II) dichloride) might act as inhibitors of SERCA. Charge displacement by SERCA adsorbed on a solid‐supported membrane was measured after ATP or Ca²⁺ concentration jumps. Our results show that KP1019, in contrast to the other metal compounds, is able to interfere with ATP‐dependent translocation of Ca²⁺ ions. An IC₅₀ value of 1 μM was determined for inhibition of calcium translocation by KP1019. Conversely, it appears that KP1019 does not significantly affect Ca²⁺ binding to the ATPase from the cytoplasmic side. Inhibition of SERCA at pharmacologically relevant concentrations may represent a crucial aspect in the overall pharmacological and toxicological profile of KP1019.     

Effect of Synthesis and Sintering Conditions on the Thermoelectric Properties of n-Doped Mg2Si

Magnesium silicide (Mg₂Si)-based alloys are promising candidates for thermoelectric (TE) energy conversion in the middle–high temperature range. The detrimental effect of the presence of MgO on the TE properties of Mg₂Si based materials is widely known. For this reason, the conditions used for synthesis and sintering were optimized to limit oxygen contamination. The effect of Bi doping on the TE performance of dense Mg₂Si materials was also investigated. Synthesis was performed by ball milling in an inert atmosphere starting from commercial Mg₂Si powder and Bi powder. The samples were consolidated, by spark plasma sintering, to a density >95%. The morphology, and the composition and crystal structure of samples were characterized by field-emission scanning electronic microscopy and x-ray diffraction, respectively. Moreover, determination of Seebeck coefficients and measurement of electrical and thermal conductivity were performed for all the samples. Mg₂Si with 0.1 mol% Bi doping had a ZT value of 0.81, indicative of the potential of this method for fabrication of n-type bulk material with good TE performance.     

Visualizing the structure and bridges of the intellectual property management and strategy literature: a document co-citation analysis

This article uses document co-citation analysis to objectively explore the underlying structure of the intellectual property research domain, taken from a managerial and strategic standpoint. The goal of this study is identifying its main research areas, understanding its current state of development and suggesting potential future directions, by analyzing the co-citations from 181 papers published between 1992 and 2011 in the most influential academic journals. Five main clusters have been identified, mapped, and labeled as follows: Economics of patent system, technological and institutional capabilities, university patenting, intellectual property exploitation, and division of labor. Their most active areas on this topic, and the most influential and co-cited papers have been identified and described. Also, intra- and inter-cluster knowledge base diversity has been assessed by using indicators stemming from the domains of information theory and biology. A t test has been performed to assess the significance of the inter-cluster diversity. The knowledge bases of these five clusters are significantly diverse, this meaning that they are five co-existing paradigms.     

Writing and Functionalisation of Suspended DNA Nanowires on Superhydrophobic Pillar Arrays

Nanowire arrays and networks with precisely controlled patterns are very interesting for innovative device concepts in mesoscopic physics. In particular, DNA templates have proven to be versatile for the fabrication of complex structures that obtained functionality via combinations with other materials, for example by functionalisation with molecules or nanoparticles, or by coating with metals. Here, the controlled motion of the a three‐phase contact line (TCL) of DNA‐loaded drops on superhydrophobic substrates is used to fabricate suspended nanowire arrays. In particular, the deposition of DNA wires is imaged in situ, and different patterns are obtained on hexagonal pillar arrays by controlling the TCL velocity and direction. Robust conductive wires and networks are achieved by coating the wires with a thin layer of gold, and as proof of concept conductivity measurements are performed on single suspended wires. The plastic material of the superhydrophobic pillars ensures electrical isolation from the substrate. The more general versatility of these suspended nanowire networks as functional templates is outlined by fabricating hybrid organic–metal–semiconductor nanowires by growing ZnO nanocrystals onto the metal‐coated nanowires.     

Mechanical behaviour and failure modes of metal to composite adhesive joints for nautical applications

In this paper, the influence of several parameters on the mechanical behaviour and failure modes of hybrid bonded joints aluminium/composite was investigated. Particularly, the effects of metallic surface condition, adhesive properties and thickness on single-lap joint resistance were analysed. To these aims, two adhesives were used and, for each adhesive, two different adhesive thicknesses (0.5 and 1.5?mm) have been investigated. Furthermore, two sets of joints for each adhesive kind and thickness were investigated: the former was obtained using aluminium blanks which were previously mechanically treated with sandpaper (P60) and the latter using aluminium treated with sandpaper and with presence of fillets in the ends of the overlap area. In order to improve the adhesion strength between polymeric adhesive and aluminium, two metal surface treatments have also been performed using a silane coupling agent, ??-glycidoxypropyltrimethoxysilane (??-GPS). The mechanical performances and failure modes were found to be significantly increased using the chemical pre-treatments with ??-GPS silane coupling agent unlike other parameters investigated. As regard the thickness of the adhesive layer, the better value is found to be equal to 0.5?mm, for both adhesives investigated.     

Study of liquid sloshing: numerical and experimental approach

In this paper, sloshing phenomenon in a rectangular tank under a sway excitation is studied numerically and experimentally. Although considerable advances have occurred in the development of numerical and experimental techniques for studying liquid sloshing, discrepancies exist between these techniques, particularly in predicting time history of impact pressure. The aim of this paper is to study the sloshing phenomenon experimentally and numerically using the Smoothed Particle Hydrodynamics method. The algorithm is enhanced for accurately calculating impact load in sloshing flow. Experiments were conducted on a 1:30 scaled two-dimensional tank, undergoing translational motion along its longitudinal axis. Two different sloshing flows corresponding to the ratio of exciting frequency to natural frequency were studied. The numerical and experimental results are compared for both global and local parameters and show very good agreement.     

Effect of chemical treatments on the mechanical and thermal behaviour of okra (Abelmoschus esculentus) fibres

The effect of a number of chemical treatments on the mechanical (tensile), thermal (FTIR, TGA) and water absorption properties of okra (Abelmoschus esculentus) fibres has been studied. These included: bare scouring of the fibres; bleaching (pure, and followed by acetylation or alkalisation); pure acetylation; sodium sulphite; sulphuric acid with potassium permanganate. Tensile properties of the fibres were studied using Weibull statistics and correcting the values obtained by the void content measured from SEM images. A separate set of SEM images allowed also characterising their failure mode. The general trend of the results shows some decrease in mechanical properties by all treatments, although the effect is partially concealed by the high variation of fibre dimensions and the relative variation of void content. Fracture surfaces present an increased level of twisting as an effect of chemical modification resulting in reduced adhesion force between the fibrils, though seldom in their physical separation. Treatments involving acetylation result in limited fibre degradation, whilst bleaching, scouring and particularly permanganate treatment, though mechanically damaging for the fibres, have lower influence on their thermal behaviour. Water absorption profile is significantly improved by all applied treatments.